Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Sowthistle yellow vein nucleorhabdovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by D.C. Stenger, 1991.

Nomenclature

Acronym

SYVV

ICTV decimal code

62.0.5.0.007

Host range and symptoms

Transmission

Transmitted by a vector; an insect; Hyperomyzus lactucae; Aphididae. Principal natural vector(s): Hyperomyzus lactucae. Not transmitted by Chaetosiphon jacobi. Transmitted in a persistent manner. Virus retained when the vector moults; transmitted congenitally to the progeny of the vector; not transmitted by mechanical inoculation; probably transmitted by grafting; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.

Experimental host range

Few (<3) families susceptible.

Susceptible host species

• Lactuca sativa
• Sonchus oleraceus

Families containing susceptible hosts

• Compositae (2/2)

Sources of host-range data

Peters (1971).

Physical and biochemical properties

Properties of particles in sap

Electron microscopy: to retain membrane, fix with glutaraldehyde and stain with neutral PTA, otherwise `bullets' are found.

Purification method

Jackson and Christie (1977).

Particle morphology

Virions rhabdo- or bullet-shaped; enveloped; usually straight; with a clear modal length; of 230 nm; 100 nm wide. Axial canal obscure. Basic helix obvious; pitch of basic helix 4.4 nm.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 1000 S. Density c. 1.18 g cm^-3 in sucrose.

Biochemical properties

Virions contain 1 % nucleic acid; 80 % protein; 19 % lipid (probably).

Genome consists of RNA; single-stranded; linear. Total genome size 13-14 kb. Genome unipartite; largest (or only) genome part 13-14 kb. Genomic nucleic acid isolated by Stenger et al. (1988). Infectivity lost when deproteinised with proteases (probably); lost when deproteinised with phenol or detergent (probably).

Features of the genome

Features of the genome: genome encodes at least 4 size classes of polyadenylated mRNA species.

Non-genomic nucleic acid not found in the virions. Sub-genomic mRNA not found in infected cells.

Features of proteins

Virion protein(s) five; M_r of the largest 200; L and it is transcriptase. M_r of 2nd largest 78; G and appear as spikes. M_r of 3rd largest 56; N, nucleocapsid. M_r of 4th largest 44; M1. M_r of 5th largest 37; M2. Method of preparation: Dale and Peters (1981). Virion proteins glycosylated; are and it is the G protein.

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in leaves, roots, phloem and companion cells; in cytoplasm, in nuclei, and in the perinuclear space. Inclusions present in infected cells; are viroplasms and unusual in shape; and many bacilliform virions found in the perinuclear space; they contain virions.

Taxonomy and relationships

Nucleorhabdovirus: Rhabdoviridae

Virus(es) with serologically unrelated virions

Sonchus yellow net virus.

Best tests for diagnosis

32p probes using cloned DNA fragments in Northern or dot blots. Bacilliform virions in Sonchus oleraceus transmitted persistently with a long latent period by Hyperomyzus lactucae.

Comments and References

References

• Dale, L.J. and Peters, D. (1981). Intervirology 16: 86.
• Jackson, A.O., Francki, R.I.B. and Zuideman, D. (1987). In: The Rhabdoviruses; ed. R.R. Wagner. Plenum Press, New York.
• Peters, D. (1971). CMI/AAB Descr. Pl. Viruses No. 62, 3 pp.
• Stenger, D.C., Richardson, J., Sylvester, E.S., Jackson, A.O. and Morris, T.J. (1988). Phytopathology 78: 1473.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 16^th January 1997.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.