Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Bean golden mosaic bigeminivirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by S. Haber and A.J. Howarth, 1983. Revised A.J. Howarth, 1990.

Nomenclature

Synonyms

virus del mosaico dorado.

Acronym

BGMV

Strains

isolates from Venezuela and Puerto Rico differ in DNA hybridization analyses.

ICTV decimal code

29.0.3.0.009

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Macroptilium lathyroides, Phaseolus vulgaris, P. lunatus, Malvastrum coromandelianum - vein yellow net, interveinal chlorosis.

Transmission

Transmitted by a vector; an insect; Bemisia tabaci (Sida race); Aleyrodidae. Transmitted in a persistent manner. Virus retained when the vector moults; does not multiply in the vector; not transmitted congenitally to the progeny of the vector; some isolates transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.

Geographical distribution

Spreads in Brazil, the Dominican Republic, Guatemala, Jamaica, Puerto Rico, and Venezuela.

Experimental host range

Few (<3) families susceptible. Experimentally infected plants mostly show chlorosis.

Diagnostically susceptible host species and symptoms

• Phaseolus vulgaris cv. Top Crop, Phaseolus lunatus - systemic vein chlorosis, golden mosaic.

Diagnostically insusceptible host species

Glycine max, Rhynchosia minima (distinguishes the virus from Rhynchosia mosaic).

Maintenance and propagation hosts

Phaseolus vulgaris cv. Top Crop.

Assay hosts (Local lesions or Whole plants)

Phaseolus vulgaris cvs Top Crop and Red Kidney (W) and Red Mexican 35 (L).

Susceptible host species

• Cajanus cajan
• Glycine max
• Macroptilium lathyroides
• Malvastrum coromandelianum
• Pachyrhizus erosus
• Phaseolus acutifolius
• Phaseolus coccineus
• Phaseolus longipedunculatus
• Phaseolus lunatus
• Phaseolus vulgaris
• Vigna angularis
• Vigna mungo

Insusceptible host species

• Glycine max
• Rhynchosia minima

Families containing susceptible hosts

• Leguminosae-Papilionoideae (11/11)
• Malvaceae (1/1)

Families containing insusceptible hosts

• Cyperaceae (1/1)
• Leguminosae-Papilionoideae (1/11)

Sources of host-range data

Galvez and Castano (1976); J. Bird (unpublished).

Physical and biochemical properties

Properties of particles in sap

TIP: 50-55 °C. LIV: 72 days. DEP: log₁₀ minus 2. Leaf sap contains few virions. Electron microscopy: aldehyde fixation essential unless PTA used at pH 4.

Purification method

Goodman et al. (1977); Goodman and Bird (1978).

Particle morphology

Virions geminate; not enveloped; 18-20 nm in diameter; dimers 30 nm in length; angular in profile; without a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 69 S. Density 1.717 g cm^-3 in CsCl (without aldehyde fixation). Density 1.31 in Cs₂SO₄. Isoelectric point pH 7.

Biochemical properties

Virions contain 20 % nucleic acid; 80 % protein; 0 % lipid.

Genome consists of DNA; single-stranded; circular. Total genome size 5.234 kb. Genome of two parts; largest (or only) genome part the larger 2.647 kb; the 2nd largest 2.587 kb. Base composition 21 % G; 28.1 % A; 18.3 % C; 32.6 % T. Infectivity decreased when deproteinised with proteases (proteinase K has no effect on protoplast infectivity); retained when deproteinised with phenol or detergent. Poly A region absent. Additional factor not required for infectivity. Genome has no tRNA-like activity. Nucleotide sequence references: Howarth et al. (1985).

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• D00200 Em(40)_vi:GEMBGACG Gb(84)_vi:MBGACG Bean golden mosaic virus (BGMV), DNA A, complete sequence. 4/90 2,585bp
• D00201 Em(40)_vi:GEMBGBCG Gb(84)_vi:MBGBCG Bean golden mosaic virus (BGMV), DNA B, complete genome. 1/91 2,647bp.
• L01635 Em(40)_vi:GEMBGCOAR Gb(84)_vi:MBGCOAREPA Bean golden mosaic geminivirus type II coat protein (AR1) gene complete cds, (AL2 and AL3)
• L01636 Em(40)_vi:GEMBGCOA1 Gb(84)_vi:MBGCOAREPB Bean golden mosaic geminivirus type II (BR1 and BL1) genes, complete cds. 11/92 2,608bp.
• L34266 Gb(84)_vi:MBGAC1A Bean golden mosaic geminivirus (clone pCR1) AC1 gene, partial cds. 7/94 222bp.
• L34267 Gb(84)_vi:MBGAC1B Bean golden mosaic geminivirus (clone pCR1) AC1 gene, partial cds. 7/94 222bp.
• L34268 Gb(84)_vi:MBGAC1C Bean golden mosaic geminivirus (clone pGA3) AC1 gene, partial cds. 7/94 222bp.
• L34269 Gb(84)_vi:MBGAC1D Bean golden mosaic geminivirus (clone pJA1) AC1 gene, partial cds. 7/94 222bp
• M10070 Em(40)_vi:GEBGMV1 Gb(84)_vi:MBGCG1Z Bean golden mosaic virus, DNA 1 of complete genome. 1/91 2,646bp.
• M10080 Em(40)_vi:GEBGMV2 Gb(84)_vi:MBGCG2Z Bean golden mosaic virus, DNA 2 of complete genome. 4/90 2,587bp.
• M88686 Em(40)_vi:GEMBGARAL Gb(84)_vi:MBGARAL Bean golden mosaic geminivirus viral coat protein (AR1) gene, complete cds; putative replicat
• M88687 Em(40)_vi:GEMBGBRBL Gb(84)_vi:MBGBRBL Bean golden mosaic geminivirus BR1 gene, complete cds and BL1 gene, complete cds. 9/92 2,580b
• M91604 Em(40)_vi:GEMBGCGA Gb(84)_vi:MBGCGA Bean golden mosaic virus coat protein gene, complete cds; putative replication protein gene
• M91605 Em(40)_vi:GEMBGCGB Gb(84)_vi:MBGCGB Bean golden mosaic virus BR1 and BL1 genes, complete cds's. 9/92 2,596bp.
• S66724 Em(40)_vi:S66724 Gb(84)_vi:S66724 (clone DR-1 hypervariable region) bean golden mosaic geminivirus, Genomic, 203 nt. 1/94 203
• S66726 Em(40)_vi:S66726 Gb(84)_vi:S66726 (clone DR-2 hypervariable region) bean golden mosaic geminivirus, Genomic, 203 nt. 1/94 203
• S66729 Em(40)_vi:S66729 Gb(84)_vi:S66729 (clone DR-3 hypervariable region) bean golden mosaic geminivirus, Genomic, 203 nt. 1/94 203
• S66730 Em(40)_vi:S66730 Gb(84)_vi:S66730 (clone DR-4 hypervariable region) bean golden mosaic geminivirus, Genomic, 203 nt. 1/94 203
• S66732 Em(40)_vi:S66732 Gb(84)_vi:S66732 (clone DR-5 hypervariable region) bean golden mosaic geminivirus, Genomic, 203 nt. 1/94 203
• S66733 Em(40)_vi:S66733 Gb(84)_vi:S66733 (DNA-B, clone pDRB-PCR hypervariable region) bean golden mosaic geminivirus, Genomic, 203 nt.

Features of the genome

Non-genomic nucleic acid not found in the virions.

Features of proteins

Virion protein(s) one; M_r 27400. Method of preparation: Goodman et al. (1980). Amino acid sequence: inferred from nucleotide sequence.

Virus-coded non-virion proteins identified by genomic sequence analysis; five proteins found. M_r of the largest 40100. M_r of 2nd largest 32700. M_r of 3rd 29600. M_r of 4th 15600. M_r of 5th and smaller 14500.

Replication

Genome replicates probably in nuclei. Coat protein mRNA translated in the cytoplasm. Replication does not depend on a helper virus.

Cytopathology

Virions found in leaves, mesophyll, phloem and stems; in nuclei and in nucleoli. Inclusions present in infected cells; are unusual in shape; hexagonally packed crystalline arrays or loose aggregates of virion-like particles in the nucleus. Other cellular changes: the occurrence of characteristic fibrillar rings in nuclei, after nucleoli undergo hypertrophy.

Taxonomy and relationships

bigeminivirus: Geminiviridae

Virus(es) with serologically related virions

Abutilon mosaic, African cassava mosaic, squash leaf curl and tomato golden mosaic viruses.

Comments and References

References

• Bird, J., Perez, J.E., Alconero, R., Vakili, N.G. and Melandez, P.L. (1972). J. agric. Univ. Puerto Rico 56: 64.
• Galvez, G.E. and Castano, M. (1976). Turrialba 26: 205.
• Goodman, R.M. (1977). Virology 83: 171.
• Goodman, R.M. and Bird, J. (1978). CMI/AAB Descr. Pl. Viruses No. 192, 4 pp.
• Goodman, R.M., Bird, J. and Thongmeearkom, P. (1977). Phytopathology 67: 37.
• Goodman, R.M., Shock, T.L., Haber, S., Browning, K.S. and Bowers, G.R. (1980). Virology 106: 168.
• Haber, S., Howarth, A.J. and Goodman, R.M. (1983). Virology 129: 517.
• Howarth, A.J. and Vandemark, G.J. (1989). J. gen. Virol. 70: 2717.
• Howarth, A.J., Caton, J., Bossert, M. and Goodman, R.M. (1985). Proc. Natl. Acad. Sci. U.S.A. 82: 3572.
• Kim, K.S., Shock, T.L. and Goodman, R.M. (1978). Virology 89: 22.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 16^th January 1997.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.